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BrightLine® single-band filter set, optimized for Qdot 605 Nanocrystals and other like fluorophores

BrightLine® single-band filter set, optimized for Qdot 605 Nanocrystals and other like fluorophores

BrightLine® single-band filter set, optimized for Qdot 605 Nanocrystals and other like fluorophores

Semrock部件编号: QD605-C-U03

Semrock

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 605 emission with minimal crosstalk from neighboring Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

比较产品
General Filter Specifications Value
Optical Damage Rating  

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications Value
Scratch-Dig  

60-40

Bandpass  

Single-band

Blocking  

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

Dichroic Beamsplitters  

Long-pass

Dichroic Flatness  

Standard Epi-fluorescence

Dichroic Orientation  

Reflective coating side should face toward light source and sample.

Edge Steepness  

Standard (for Fluorescence)

General Filter Specifications  

Value

Microscope Compatibility  

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Optical Specifications  

Value

Physical Filter Specifications  

Value

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 430 431 541
Cascade Yellow™ 399 549
Di-8-ANEPPS 467 631
DiA 457 586
FM 1-43 473 579
Lucifer yellow 428 544
mHoneyDew 478 562
Pacific Orange 400 551
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
SYPRO Ruby protein gel stain 469 631
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  • Image
  • SKU
  • Price
  • Optical Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • General Filter Specifications
  • Angle of Incidence
  • Cone Half-angle
  • Optical Damage Rating
  • Physical Filter Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • Transverse Tolerance
  • Clear Aperture
  • Scratch-Dig
  • Substrate Type
  • Orientation
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