021-52415599

BrightLine® single-band laser filter set, optimized for 561 & 568 nm laser sources

Semrock部件编号: LF561-B-000

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.Our improved version is certified to deliver dichroic flatness

比较产品

Optical Specifications	Value
Laser Wavelength 1 high	568.2 nm
Laser Wavelength 1 low	561.4 nm
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

0 total selected Clear selection

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
2-dodecylresorufin-lipid	582	595
5-ROX (carboxy-X-rhodamine)	578	604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)	542	568
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor® 546	556	572
Alexa Fluor® 555	553	565
Alexa Fluor® 568	579	603
Alexa Fluor® 594	590	618
Amplex UltraRed peroxidation product (pH 7.5)	568	581
AsRed 2	576	592
ATTO 550	553	576
ATTO 565	563	592
ATTO 590	594	624
BOBO™-3	570	605
BODIPY TMR-X	544	573
BODIPY TR-X (MeOH)	544	573
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Calcium Orange	589	609
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
Cy3.5™	578	591
Cy3™	554	566
Dendra2 (Red)	554	569
DiI	551	569
DsRed	559	583
DsRed-Express	556	584
dTomato	556	582
DY-590	582	599
DyLight 549	553	569
DyLight 594	593	618
Ethidium bromide	518	603
Ethidium homodimer	527	617
FluoSpheres Red fluorescent microspheres	577	607
HcRed1	588	618
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
Hypericin	600	603
KFP-Red	575	599
LOLO-1	568	580
LysoTracker Red	573	592
Magnesium Orange	550	574
mApple	566	594
mCherry	587	610
Merocyanine 540	559	579
MitoTracker™ Orange	551	575
MitoTracker™ Red	578	598
mKate2	588	633
mOrange2	551	565
mPlum	589	649
mRFP	555	583
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
mTangerine	568	585
Nile red-phospolipid	553	637
OFP	547	567
Orange 552	552	574
pHrodo™, succinimidyl ester	560	587
Pro-Q Diamond	556	583
Propidium Iodide (PI)	305	617
Qdot® 585 Nanocrystals	300	588
Qdot® 605 Nanocrystals	300	602
Qdot® 625 Nanocrystals	300	621
R-phycoerythrin (PE)	565	576
ReAsH-CCPGCC	592	606
Red 580	580	603
Resorufin	571	584
Rhod-2	553	577
Rhodamine phalloidin	558	575
Rhodamine Red-X	573	591
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 6.0)	548	586
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 6.0)	549	587
SNARF-1 514nm (ph 9.0)	576	638
SpectrumOrange	554	587
Sulforhodamine 101-EtOH	578	593
SYPRO Ruby protein gel stain	469	631
Tetramethylrhodamine dextran	554	582
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red®	592	614
TRITC (Tetramethylrhodamine)	552	578
TurboFP635(Katushka)	591	638
TurboRFP	553	573
X-Rhod-1 Indicator	580	601
YOYO-3	611	631