021-52415599

BrightLine® single-band laser filter set, optimized for 405 nm laser sources

Semrock部件编号: LF405-B-000

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.Our improved version is certified to deliver dichroic flatness

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比较产品

Optical Specifications	Value
Edge Wavelength 1	405 nm
Center Wavelength 1	375 nm
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

0 total selected Clear selection

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor® 350	343	441
Alexa Fluor® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
CFP (ECFP)	433	475
DAPI	359	461
DEAC	432	472
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437