Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings. This 9-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels. Interested in learning how this filter set will perform with your light source? Try Semrock’s SearchLight spectral plotting & analysis tool.