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BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

Semrock部件编号: FRET-GFP/RFP-D-NTE

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field micro

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Bandpass  

Single-band
Dichroic Beamsplitters  

Long-pass
Dichroic Flatness  

Standard Epi-fluorescence
Edge Steepness  

Standard (for Fluorescence)

FRET-GFP/RFP-D-OMF-ZERO

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FRET-GFP/RFP-D-ZHE-ZERO

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FRET-GFP/RFP-D-000

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FRET-GFP/RFP-D-OFF-ZERO

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Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 488 499 520
Cy2™ 492 507
FITC (Fluorescein) 495 519
GFP (EGFP) 489 511
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  • Image
  • SKU
  • Price
  • Optical Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • General Filter Specifications
  • Angle of Incidence
  • Cone Half-angle
  • Optical Damage Rating
  • Physical Filter Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • Transverse Tolerance
  • Clear Aperture
  • Scratch-Dig
  • Substrate Type
  • Orientation
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