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IDEX部件编号: FL-004432
Individual fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. All thin-film, hard-coated construction for unsurpassed performance and reliability.
| Optical Specifications | Value |
|---|---|
| Transmission Band 1 |
Tavg > 93% 475 – 495 nm |
| Blocking Band 2 |
ODavg > 5 275 – 460 nm |
| Blocking Band 4 |
ODavg > 5 573 – 925 nm |
| Blocking Band 1 |
ODavg > 5 200 – 275 nm (Design specification – measurements are noise-floor limited). |
| Blocking Band 3 |
ODavg > 10 510 – 556 nm (Design specification – measurements are noise-floor limited). |
| Center Wavelength 1 |
485 nm |
| FWHM Bandwidth 1 (nominal) |
24.9 nm |
| Guaranteed Minimum Bandwidth 1 |
20 nm |
| General Filter Specifications | Value |
| Angle of Incidence |
0 ± 5 degrees |
| Cone Half-angle |
7 degrees |
| Optical Damage Rating |
Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours. |
| Physical Filter Specifications | Value |
| Transverse Dimensions (diameter) |
25 mm |
| Transverse Tolerance |
+ 0.0 / – 0.1 mm |
| Filter Thickness (mounted) |
5.0 mm |
| Filter Thickness Tolerance (mounted) |
± 0.1 mm |
| Filter Thickness (unmounted) |
2.0 mm |
| Filter Thickness Tolerance (unmounted) |
± 0.1 mm |
| Clear Aperture |
≥ 21 mm |
| Scratch-Dig |
60-40 |
| Orientation |
Arrow on ring is preferred direction of propagation of light |
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
