021-52415599

BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

Semrock部件编号: DAPI-50LP-A-000

This unique “flamethrower” filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For most applications that require minimized photobleaching, we recommend our DAPI-11LP-A set. In all cases our filters are uniquely hard-coated, for “no burn-out” performance where it is most needed.

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General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

0 total selected Clear selection

DAPI-50LP-A-ZHE

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DAPI-50LP-A-OMF

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DAPI-50LP-A-NQF-ZERO

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DAPI-50LP-A-OFF-ZERO

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Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-amino-4-methylcoumarin (AMC)	346	442
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor® 350	343	441
Alexa Fluor® 405	401	422
Alexa Fluor® 430	431	541
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BD Horizon V500	410	500
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
Cascade Yellow™	399	549
CellTrace calcein violet	400	452
CellTracker Violet BMQC+GSH	406	526
ChlorophyllA	480	650
DAPI	359	461
Dapoxyl	373	579
DEAC	432	472
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 350	351	434
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
Fluoro-Gold™	365	418
FluoSpheres Blue fluorescent microspheres	356	414
Fura Red™ (high pH)	572	657
Fura-2 (calcium bound)	336	505
Fura-2 (calcium free)	366	515
GFPuv	395	509
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
Hypericin	600	603
indo-1	346	479
LIVE-DEAD Fixable Aqua Dead Cell Stain	367	526
LIVE-DEAD Fixable Blue Dead Cell Stain	343	441
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
Lucifer yellow	428	544
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
Pacific Orange	400	551
Qdot® 545 Nanocrystals	300	543
Qdot® 565 Nanocrystals	300	564
Qdot® 585 Nanocrystals	300	588
Qdot® 605 Nanocrystals	300	602
Qdot® 625 Nanocrystals	300	621
Qdot® 655 Nanocrystals	300	654
Qdot® 705 Nanocrystals	300	702
Qdot® 800 Nanocrystals	300	792
Quinine sulfate	349	461
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
SYPRO Ruby protein gel stain	469	631
TagBFP	402	457
ThiolTracker Violet+GSH	404	526
Vybrant DyeCycle Violet	369	437