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Semrock’s award-winning blue-green-red multiband filter set is uniquely optimized to provide simultaneously stunning brightness, striking contrast, and superb color balance when viewing multiple fluorophores by eye or with a high-quality color CCD camera. The result is brilliant colors and a black background.
| General Filter Specifications | Value |
|---|---|
| Optical Damage Rating |
Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours. |
| Physical Filter Specifications | Value |
| Scratch-Dig |
60-40 |
| Bandpass |
Multiband |
| Blocking |
BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design. |
| Dichroic Beamsplitters |
Multiedge |
| Dichroic Flatness |
Standard Epi-fluorescence |
| Dichroic Orientation |
Reflective coating side should face toward light source and sample. |
| Edge Steepness |
Standard (for Fluorescence) |
| General Filter Specifications |
Value |
| Microscope Compatibility |
BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes. |
| Optical Specifications |
Value |
| Physical Filter Specifications |
Value |
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| DAPI | 359 | 461 |
|
|
| DEAC | 432 | 472 |
|
|
| DY-415 | 418 | 470 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| Hoechst 33258 | 352 | 455 |
|
|
| Hoechst 33342 | 352 | 455 |
|
|
| Hoechst 34580 | 392 | 440 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| POPO-1 | 433 | 457 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| TagBFP | 402 | 457 |
|
