021-52415599

BrightLine® single-band filter set, optimized for Cyan Fluorescent Protein and other like fluorophores

Semrock部件编号: CFP-2432C-000

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring CFP and its variants – no more compromising or exchanging filter sets for different CFP experiments. For CFP-YFP FRET measurements, consider our economical FRET combination set FRET-CFP/YFP-A, or our high-performance “Sedat” filter set CFP/YFP-2X2M-A.

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General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

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CFP-2432C-NTE

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CFP-2432C-ZHE-ZERO

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CFP-2432C-OMF

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CFP-2432C-OFX

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Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
Acridine Yellow	470	550
AmCyan1	458	489
Aqua 431	430	478
ATTO 425	436	484
ATTO 465	453	507
BD Horizon V500	410	500
BOBO™-1	461	484
Cerulean	434	473
CFP (ECFP)	433	475
DEAC	432	472
DY-415	418	470
ECFP	435	475
evoglow-Bs1	449	496
LysoSensor Green	448	502
mBBr+GSH	394	490
mTFP1	462	492
PO-PRO-1	433	456
POPO-1	433	457
SpectrumAqua	434	481
SYTO 45	451	485
SYTOX Blue	444	470
TagCFP	458	480