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BrightLine 图像分离二向色镜分束镜

2020年9月30日 作者: IDEX Health & Science

Semrock 的用于图像分离的二向色镜分束镜符合该应用的行业要求,支持同时多色成像应用,如 SRM 高分辨率显微镜、FRET(荧光共振能量转移)等,亦可用于为常规荧光应用提供好的平整度、宽的阻挡范围、大的视野。

image splitting dichroic beamsplitters

diagram illustrating a camera setup with an image-splitting dichroic beamsplitter

compare flatness of image-splitting dichroic beamsplitter to standard dichroic beamsplitter

对于图像分离的应用,如果平整度不够,会导致显著的光学畸变,损坏图像的分辨率和信噪比。欲深入了解此内容,请阅读我们的技术文章:

BrightLine® 图像分离二向色镜分束镜 – 用于 SRM 高分辨率显微镜

  • λ/5 P-V 反射波前差(RWE),厚度 3 mm
  • 优化了光谱边缘,适用于常用荧光蛋白组合的成像
  • 大尺寸的成像光束,适用于 SRM 高分辨率显微镜
  • 厚的基材,提供了好的装配解决方案
  • 宽的反射带 – 深紫外 UV & 宽的透射区域 – 远红外 IR 至1200 nm     
Image-splitting dichroic beamsplitters for super-resolution microscopy

compare transmission of 9 image-splitting dichroic beamsplitters for super-resolution microscopy

平整度 / RWE 反射波前差分类

应用举例

标明
曲率半径

Maximum
反射光束直径 mm

反射
波前差 @
632.8 nm, PV

该产品系列的
产品型号示例

图像分离 在基于像素的探测器上, 分离发射光的荧光信号 ~ 1275 米 37 <0.2λ BrightLine 图像分离
(FF509-FDi02-t3-)

 

分离的
荧光蛋白组合

反射带 (nm)

透射带 (nm)

产品型号  

 库存状态

DAPI / FITC
BFP / GFP

350 – 475 492.3 – 1200 FF484-FDi02-t3-25×36 库存现货

CFP / YFP

350 – 500 518.3 – 1200 FF509-FDi02-t3-25×36 库存现货

GFP / mOrange

350 – 528.4 547.7 – 1200 FF538-FDi02-t3-25×36 库存现货

YFP / dTomato

350 – 550 570.1 – 1200 FF560-FDi02-t3-25×36 库存现货

GFP / mCherry
FITC / TexasRed

 350 – 570 590.8 – 1200  FF580-FDi02-t3-25×36 库存现货

Cy3 / Cy5

350 – 629.5 652 – 1200 FF640-FDi02-t3-25×36 库存现货

TexasRed / Cy5

350 – 650 673.7 – 1200 FF662-FDi02-t3-25×36 库存现货

iRFP670 / iRFP720

350 – 686 712 – 1200 FF699-FDi01-t3-25×36 库存现货

Cy5.5 / Cy7

400 – 722 754 – 1200 FF738-FDi01-t3-25×36 库存现货

Image-splitting dichroic beamsplitters for standard microscopyBrightLine® 图像分离二向色镜分束镜 – 用于常规显微镜

  • 推荐用于常规的图像分离的荧光应用 
  • 优化了光谱边缘,适用于常用荧光蛋白组合的成像
  • 宽的反射带 – 深紫外 UV & 宽的透射区域 — 远红外 IR 至950 nm

compare transmission of 9 image-splitting dichroic beamsplitters for standard microscopy

 

平整度 / RWE 反射波前差分类

应用举例

标明
曲率半径

Maximum
反射光束直径 mm

反射
波前差 @
632.8 nm, PV

该产品系列的
产品型号示例

图像分离 在基于像素的探测器上, 分离发射光的荧光信号 ~ 100 米 10 < 2λ BrightLine 图像分离
(FF509-FDi01-)

 

分离的
荧光蛋白组合

反射带 (nm)

透射带 (nm)

产品型号  

 库存状态

DAPI / FITC
BFP / GFP

350 – 475 492.3 – 950 FF484-FDi01-25×36 库存现货

CFP / YFP

350 – 500 518.3 – 950 FF509-FDi01-25×36 库存现货

GFP / mOrange

350 – 528.4 547.7 – 950 FF538-FDi01-25×36 库存现货

YFP / dTomato

350 – 550 570.1 – 950 FF560-FDi01-25×36 库存现货

GFP / mCherry
FITC / TexasRed

 350 – 570 590.8 – 950  FF580-FDi01-25×36 库存现货

Cy3 / Cy5

350 – 629.5 652 – 950 FF640-FDi01-25×36 库存现货

TexasRed / Cy5

350 – 650 673.7 – 950 FF662-FDi01-25×36 库存现货

iRFP670 / iRFP720

350 – 686 712 – 1200 FF699-FDi01-t1-25×36 库存现货

Cy5.5 / Cy7

400 – 722 754 – 1200 FF738-FDi01-t1-25×36 库存现货

点击下载 the Brightline® 图像分离二向色镜分束镜产品参数表

Select the fields to be shown. Others will be hidden. Drag and drop to rearrange the order.
  • Image
  • SKU
  • Price
  • Optical Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • General Filter Specifications
  • Angle of Incidence
  • Cone Half-angle
  • Optical Damage Rating
  • Physical Filter Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • Transverse Tolerance
  • Clear Aperture
  • Scratch-Dig
  • Substrate Type
  • Orientation
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