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Semrock 滤光片

Semrock 滤光片

分离发射信号

在荧光显微镜中,有多种可供选择的方案用于查看单个样品的多种颜色。使用二向色镜分束镜分离发射信号的主要好处是,能够同时而不是按顺序查看多种颜色。这种使用二向色镜分离发射信号的技术在流量仪器中是标准的,但在使用荧光显微镜时需要特定的安排。

当使用非像素化检测器(如光电倍增管 PMT )收集发射信号时,任何具有适当透射和阻挡特性的二向色镜都是可以接受的。对于使用像素化检探测器(如 CCD)成像的信号,需要显着考虑平整度的要求。

Semrock 的 图像分割二向色镜分束镜 可有效分离多色发射信号,同时保持出色的图像。这些二向色镜分束镜设计方便,入射角为 45°,可用于许多流行的荧光团组合。它们的宽反射和透射带以及平坦度可以捕捉光线,同时减少图像像差。使用我们种类繁多的 带通和长通发射滤光片,来阻挡激发光并减少渗漏。

了解关于分离发射信号的更多信息:

白皮书

二向色分光镜的平整度影响焦点和图像质量

用于基于激光的荧光显微镜的滤光片

相关出版物

实现荧光图像的对齐 2005 年 8 月的 BioPhotonics 杂志

荧光成像:滤光片优化了基于激光的荧光成像系统 2005 年 8 月的 BioPhotonics 杂志

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